Tuesday, Oct 20, 2014
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Introducing PacBio RS II: Sequencing system with industry's highest consensus accuracy and longest read lengths

Pacific Bioscience® PacBio RS II system

Recent advancements in our understanding of biological complexity have propelled the development of new tools. In the field of DNA sequencing, next-generation sequencers have dramatically increased productivity and provided novel insights into the structure and function of the genome. Despite these advances, researchers continue to face challenges in finishing genomes, characterizing variations, and understanding the function of key biological markers that may affect human health.
The PacBio RS II sequencing technology resolves single molecules in real time, allowing observation of structural and cell type variation not accessible with other technologies. These unique capabilities of the PacBio RS II system are ideally suited for a variety of applications, from De Novo assembly and targeted sequencing to detecting base modifications.
This novel approach to DNA sequencing can improve scientists' ability to understand health and disease processes, provide new mechanisms to diagnose and treat medical conditions, and enhance efforts to improve the world's food and energy supply. As more scientists adopt the SMRT technology, we expect numerous other applications of sequencing to become feasible for the first time.

Sequencing with the PacBio RS II system based on single molecule, real-time (SMRT) technology offers abundant benefits, especially:
  • Extraordinarily long reads: Produce reads with average lengths of 4,200 to 8,500 bp, with the longest reads over 30,000 base pairs.
  • Extremely high accuracy: Perform de novo assembly of genomes and detect variants with greater than 99.999% accuracy. Sequence individual molecules with 99% accuracy at greater than Sanger lengths.
  • Exquisite sensitivity: Detect minor variants that are present at a frequency less than 0.1%
For research in epigenetics and methylation, the PacBio RS II is the only commercially available system that can:
Discover a broad spectrum of DNA base modifications: Go beyond 5-methylcytosine and detect N6-methyladenine, N4-methylcytosine, DNA oxidative damage and other modified bases in the same sequencing run that reads the canonical base sequence.

Additionally, the PacBio RS II offers:
  • Shortest run time: Sequence for as little as 30 minutes and still get reads longer than Sanger sequencing
  • Least GC bias: Sequence through regions of extremely high or low GC content with ease, resulting in more even coverage
  • No amplification bias: Samples need not be amplified, improving coverage uniformity and avoiding artifacts.

Major Applications
  • De Novo Assembly including:
- Hybrid assembly
- Scaffolding
- De novo assembly with only RS II data
- Gap Filling
  • Targeted sequencing
  • Base modification identification (e.g. Methylation)
  • Isoform sequencing
Major Applications
  • Full length transcript sequencing without assembly
  • Repeat Expansions
  • Structural Variation and Copy-Number Variants
  • Compound mutations and Haplotype Phasing
  • Full-Length 16S sequencing
  • SNP detection and validation Minor Variants and Quasispecies

Latest Brochure of PacBio RSII for application-specific information

Original brochure for information of SMRT technology

For more information, please contact us or visit pacificbiosciences.com

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