Cryptic Insect Microbiome Compositions Unveiled with Full-Length 16S Sequencing

Authors: Charles Mason, Thorsten Hansen, Nicholas Newell, Scott Geib

Illustrates “the benefit of full-length 16S rRNA sequencing in improving sample resolution compared to V4 which provides new insight into the gut microbiome community composition of an invasive insect” 

Using iconPCR with autonormalization and Kinnex kits on the Revio system for sequencing of gut microbiome of Mediterranean fruit fly collected across a nine-month sampling period 

Finding “substantial differences in bacterial compositions across different medfly cohorts when sampling the full-length amplicons which were not detectible when only considering the V4 regions.” 

“long-read sequencing of full-length 16S rRNA amplicon uncover ecologically important interactions between host and gut microbiomes”

READ FULL ARTICLE: https://www.biorxiv.org/content/10.64898/2026.01.22.701149v1.full 

HIFI Systems

Genomes: Complete + accurate genomes

Transcriptomes: Full-length RNA isoforms

Epigenomes: Directly phased methylomes

Metagenomics: Species-level resolution for profiling and assembly

Targeted Sequencing: Comprehensive variant calling for regions of interest

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