Gene Company Ltd
Wednesday, Mar 19, 2014
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ScripSeq™ RNA-Seq Library Construction series

Epicentre® ScriptSeq™ Series, v2 RNA-Seq Library preparation, complete & complete gold kit


ScriptSeq schematic overview click to enlarge
ScriptSeq v2 RNA-Seq Library preparation
The ScriptSeq™ v2 RNA-Seq Library Preparation Kit* is suitable for preparing RNA-Seq libraries from rRNA-depleted or poly(A)-enriched RNA from any animal, plant, or bacterial species.
  • Requires just 500 pg to 50 ng of rRNA-depleted RNA or poly(A)+ RNA.
  • Makes cluster-ready libraries in 4 hours without adaptor ligation.
  • Enables directional, paired-end sequencing using Illumina® TruSeq™ Cluster kits and sequencing primers.
  • Produces libraries from intact, partially degraded, and FFPE RNA samples.
Index/barcode-capable (ScriptSeq™ Index PCR Primers (1-12)) are available separately

ScripSeq series are sold as Complete kit or individual library preparation kits
Complete kit included Ribo-Zero™ Gold rRNA removal reagent or Ribo-Zero™ rRNA removal reagent and ScriptSeq™ v2 RNA-Seq Library Preparation Kit. Ribo-Zero™ rRNA removal reagent remove cytomplasmic rRNA to improve RNA-Seq result. Ribo-Zero™ Gold rRNA removal reagent remove mitochrondrial rRNA and cytomplasmic, see here for application notes on Ribo-Zero.

Golbin-Zero™ Gold removes globin mRNAs and the cytoplasmic and mitochondrial rRNAs found highly abundant in blood samples.

All kits require use of Epicentre FailSafe™ PCR Enzyme Mix (provided by user) for best performance.

Sample Type Kit Input RNA Ribo-Zero version
Poly(A)-selected or rRNA-depleted RNA (provided by the user) from any source including FFPE tissue ScriptSeq™ v2 RNA-Seq Library Preparation Kit 500 pg - 50 ng of poly(A)-selected or rRNA-depleted RNA (provided by the user) No Ribo-Zero rRNA removal reagent included
Human/Mouse/Rat and other higher vertebrates; FFPE RNA ScriptSeq™ Complete Gold Kit 100 ng - 5 μg of total RNA Ribo-Zero™ Gold
Blood ScriptSeq™ Complete Gold Kit (Blood) 100 ng - 5 μg of total RNA Goblin-Zero™ Gold
Microbiome, bacterial infected human/mouse/rat ScriptSeq™ Complete Gold Kit (Epidemiology) 100 ng - 2.5 μg of total RNA Ribo-Zero™ Gold
Bacteria ScriptSeq™ Complete Kit (Bacteria) 100 ng - 5 μg of total RNA Ribo-Zero™
Plant leaf ScriptSeq™ Complete Kit (Plant Leaf) 100 ng - 5 μg of total RNA Ribo-Zero™
Plant seed or root ScriptSeq™ Complete Kit (Plant Seed/Root) 100 ng - 5 μg of total RNA Ribo-Zero™
Yeast ScriptSeq™ Complete Gold Kit (Yeast) 100 ng - 5 μg of total RNA Ribo-Zero™ Gold

For more information, please contact us or visit epibio.com

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