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Multispectral Imaging Flow Cytometry
Amnis is proud to offer the ImageStream system, a single platform that combines the analytical power of quantitative cell imaging with the statistical power of flow cytometry.
With the ImageStream system, you can:
- Quantitate the amount, location and movement of target molecules on, in or between cells
- Accurately measure cellular processes such as translocation, colocalization, reverse signaling, cell conjugate formation, cell morphology, FISH, Geneion and many more
- Generate and analyze statistically significant data on individual cells, entire populations and sub-populations
- Unambiguously confirm your gating strategies by direct visual analysis of gated cells
Leading Edge Technology Produces Unprecedented Imagery
In the ImageStream, cells are illuminated with both a brightfield source, a 488 nm laser and up to two additional lasers as optional illumination sources. The resulting composite images are spectrally decomposed into as many as 6 component images and captured using Time Delay Integration technology on a proprietary CCD camera. You see a brightfield, darkfield and up to 4 fluorescence images of every cell, while over 35 morphological and signal intensity features are calculated for every image – over 200 features in total.
Software designed for the Biologist
The IDEAS™ analytical software package provides you with powerful, yet flexible and accessible data analysis. IDEAS evaluates nearly 200 features for every cell, including multiple morphologic and fluorescence intensity measurements. These features -- and virtually combination of them -- can be used to define and characterize your cell populations. You’ll find full graphical representation and statistical reporting capabilities. Standard cytometry analyses such as gating and backgating, are accomplished with a few clicks. In the displays, your image data and graphic outputs are completely integrated – every dot on a scatterplot is linked directly to a set of cell images. Yet with all its power, IDEAS software is remarkably easy to learn and use. Most operations are intuitive and require no prior experience with image analysis software.
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