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 The MycoAlert Mycoplasma Detection Kit is designed to detect mycoplasma contamination in cell cultures. The MycoAlert Assay is simple to use, is very fast to perform, and gives reliable results, even with very low levels of mycoplasma contamination. The speed, convenience, and reliability of the assay now make mycoplasma detection so easy that it can be performed at every cell passaging to ensure that your cultures are clean and your results are untainted.
The MycoAlert Assay is a selective biochemical test that exploits the activity of mycoplasmal enzymes. The presence of these enzymes provides a rapid screening procedure allowing sensitive detection of contaminating mycoplasma in a text sample. The viable mycoplasma are lysed and the enzymes react with the MycoAlert Substrate catalysing the conversion of ADP to ATP. By measuring the level of ATP in a sample both before and after the addition of the MycoAlert Substrate, a ratio can be obtained which is indicative of the presence or absence of mycoplasma.
Fast
Get results in less than 20 minutes to quickly access the health of your cultures. Other methods take several hours to weeks to get results.
Convenient
Simply mix two reagents with 100? of your culture supernatant and take two readings on your luminometer.
Universal
Detect all mycoplasma and acholeplasma infections commonly contaminating cell cultures.
Sensitive
Detect <50 cfu/ml mycoplasma and avoid false negative results often encountered with PCR and fluorescent staining procedures.
Complete
Each kit contains all of the reagents needed to perform the assay, unlike commercial PCR kits, which require you to supply Taq Polymerase, nucleotides, restriction enzymes, and agarose gel electrophotesis reagents.
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