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xMAP technology developed by Luminex has become an assay platform around which numerous assay kits have been designed. This open architecture technology uses fluorescently labeled microspheres as a solid surface to attach specific reagents for bioassays. The attached reagents then serve to bind and capture analytes from heterogeneous samples. These bioassays are performed in 96-well filter bottom microplates, which serve as fluid reservoirs for the test reagents. In order to remove unbound material from the assay without the loss of the microspheres, vacuum is applied to the filter bottom plates, drawing liquids and unbound materials through the porous membrane leaving the microspheres behind. Here we describe the use of the ELx50 Filter Microplate Washer to automate the wash procedures required for xMAP assays.
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